TEACHING MATERIALS: Relevant Literature

Designing RNA-Seq experiments

  • RNA-seq differential expression studies: more sequence, or more replication? (Download PDF)
  • Calculating Sample Size Estimates for RNA Sequencing Data (Download PDF)
  • Sequencing depth and coverage: key considerations in genomic analyses (Download PDF)
  • Evaluation of the coverage and depth of transcriptome by RNA-Seq in chickens (Download PDF)

Wet lab

  • Quantitative Real-Time PCR-Based Analysis of Gene Expression (Download PDF)
  • Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2-ΔΔCT Method (Download PDF)

Bioinformatics–Processing RNA-Seq data

Bioinformatics–Downstream analyses with RNA-Seq data

Bioinformatics–RNA-Seq in the classroom

  • Authentic Research Experience and “Big Data” Analysis in the Classroom: Maize Response to Abiotic Stress (Download PDF)
  • Killing Two Birds with One Stone (Download PDF)