TEACHING MATERIALS: Expert Lectures: Ross Nehm, Ph.D.
Ross Nehm, Ph.D., Associate Professor, Center for Science and Math Education, Stony Brook University
Recorded during 2014 retreat
Designing RNA-Seq experiments
Illumina TruSeq RNA libraries: Part I–Start prep
Illumina TruSeq RNA libraries: Part II–Continue prep
Processing raw sequencing reads
Understanding NGS sequencing files
RNA-Seq analysis pipeline: TUXEDO protocol
RNA-Seq for differential gene expression: the tuxedo suite
RNA-Seq experimental design, quality control and analysis of differential gene expression I
RNA-Seq experimental design, quality control and analysis of differential gene expression II
Part I: Aligning and visualizing RNA-Seq data with TopHat and IGV
Part II: Quantifying transcripts with CuffLinks
Part III: Determining differential gene expression; p- and q-values
Part IV: Alternative RNA-Seq data analysis pipelines; Sailfish and Kallisto
Validating RNA-Seq Data by qRT-PCR
Biological networks, Cytoscape and BiNGO
Dickens to DNA: Introduction to de novo sequence assembly
The ENCODE project
Advanced data analysis: Command line usage
Applications in SMRT sequencing: Using long reads to understand the transcriptome
Demo: Small RNA isolation from total RNA
Genome complexity and epigenetic regulation
Best practices for STEM curriculum development
Initiating, developing and maintaining NGS skills while working with undergraduate students–a case study